Search results for "Water-in-oil droplets"

showing 2 items of 2 documents

Natural or synthetic nucleic acids encapsulated in a closed cavity of amphiphiles

2013

In this review some aspects of the interactions of organized structures of amphiphiles with natural or synthetic DNAs are briefly considered. In particular DNAs encapsulated in closed cavities of amphiphiles, specifically giant vesicles and water-in-oil droplets and reverse micelles, are dealt with. Two main applications of giant vesicles are reviewed in detail, namely their use as microreactors where reactions can be followed by optical microscopy on a single vesicle and in synthetic biology as protocell models or as potential semi-synthetic ‘‘living’’ cells. Water-in-oil droplets uses for rapid and relatively low-cost DNA amplification by PCR reaction are described as well as for in vitro…

ProtocellAqueous solutionChemistryGeneral Chemical EngineeringVesicleNanotechnologyGeneral ChemistrymicroreactorsMicellepolynucleotides in water-in-oil dropletsSynthetic biologyDNA model polynucleotides giant vesicles Reverse micellesPolynucleotideAmphiphileBiophysicsNucleic acidmicroreactors; polynucleotides in giant vesicles; polynucleotides in water-in-oil dropletspolynucleotides in giant vesiclesRSC Advances
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Towards bioarrays of cellular-like compartments for monitoring few molecular binding events

2016

The aim of this work is to artificially reproduce scalable cellular-like compartments on a chip, thus realizing specialized small volume systems to study the behaviour of interacting biomolecules by few binding events. In particular, we show an unprecedented solution-based protein-binding assay based on arrays of oil-confined water droplets containing protein targets, labelled ligands and other compounds.

Water-in-oil droplets inkjet printing Raster Image Correlation SpectroscopySettore CHIM/01 - Chimica Analitica
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